FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004976491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21326152
Sequences flagged as poor quality0
Sequence length35-231
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG7682713.602482998339316No Hit
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA6755193.167561592921217No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA5279582.475636486132144No Hit
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT5265182.468884213148251No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG4887432.2917542742825803No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG4738872.2220933246654155No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG4350322.0398991810618248No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT3892101.8250362278201897No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT3530521.6554885288260162No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA3414111.600902966461085No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG3134691.4698807361027906No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC3115041.4606666969268531No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG3092451.4500740686833706No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA3083171.4457226038715283No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT2998771.4061467816603765No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG2941321.3792080259017192No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA2809001.3171621397052782No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT2787531.3070946882494319No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC2696031.264189620330944No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT2658601.2466383996512826No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA2583441.211395285938129No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG2543721.1927702662908901No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA2505291.174750137765125No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT2490831.1679697303104657No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT2440171.1442148588268526No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT2437591.143005076583905No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT2382211.1170369600666825No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT2366051.1094594092736467No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG2337921.09626903156275No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA2125690.9967527193841628No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG2094290.9820290130165067No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG1985920.9312134697342492No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT1966060.9219009599106299No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC1805580.846650628767909No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG1761040.8257654733024503No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA1722490.807689075835153No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG1578910.7403632872915845No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT1509480.7078070155366051No Hit
TCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGA1496240.7015986756541921No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT1436190.6734407594956652No Hit
GAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGG1430620.6708289427928676No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT1392080.6527572344040312No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG1383400.6486871142998513No Hit
GGGAACCACTAGTTCTTTCAGATGGTATTCTTCAGACTATAGAAGGAGCT839910.39384038902095414No Hit
ACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGATTATTTTTTAAA837190.3925649596795522No Hit
ATTCTTCAGACTATAGAAGGAGCTTCCAGTTGAATTCACCAGTGGACAAA712570.33412966389810966No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC690830.3239356073238154No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT678550.31817741897366203No Hit
CACCATTGGCTCCTGTTTGAAATGAGCAGGCACTCCTTGGAGCAAAAGCC592390.2777763189533677No Hit
ATGAGCAGGCACTCCTTGGAGCAAAAGCCCACTGACGCTCCACCGAAAGA488800.22920215517548598No Hit
CACTGGAACTGGAGGACCCGTCTTCTGGGCTGGGTGTGACCAAGCAGGAT392530.18406039683108327No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC352210.1651540324761823No Hit
CAACATGACAGAGGACCATGTGATGCACCTGCTCCAGAATGCTGACCCCC316560.14843746776258557No Hit
TAGAAAGCTGTCTCCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAG296000.1387967224466936No Hit
GAGTAAAAAATGCGAAGAAAAGAATAGAGAAGATAGGGAAATTAGAAGAT268620.12595802562037445No Hit
GCAACAGGTGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGC224390.10521823158720804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)