FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21326152
Sequences flagged as poor quality0
Sequence length35-71
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA771300.062.50213617
GAGTCCG454100.062.35315717
AATGGAG774850.062.16800313
AGTCCGA255050.061.50283418
ATAGGAG488900.061.35253513
GGAGTCC1708000.061.27076316
GAGTCCT1474450.061.15441517
AGAGTCC412550.061.10348516
ATTGGAG771000.060.8447213
AATCGAG273400.060.76518613
AGTCCAG599250.060.7614218
AAAGGAG759400.060.7390513
TGAGTCC207000.060.49262216
CGAGTCC549000.060.2813816
ATGGAGT473850.060.21293314
TCGAGTC306800.059.91403615
AGGAGTC682650.059.7572615
TGGAGTC902700.059.66134615
AAACGAG310250.059.46639313
TAATGGA262200.059.31775312
Produced by FastQC (version 0.11.5)