FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18968462
Sequences flagged as poor quality0
Sequence length35-71
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA673250.062.6449117
GAGTCCG386150.061.65162317
GGAGTCC1439850.061.44614816
ATAGGAG409950.061.31573513
AGTCCGA209700.060.97429718
GAGTCCT1251700.060.82653817
AATCGAG238750.060.555113
ATTGGAG658350.060.38578813
AGAGTCC361400.060.34601616
AAAGGAG660600.060.0777113
AATGGAG677500.059.9532313
TCGAGTC261250.059.8280215
TGAGTCC180500.059.64545416
AGGAGTC575550.059.2668715
CGAGTCC482700.059.2496716
AGTCCAG541800.059.1805718
TAATGGA227700.058.8801412
ATCGAGT136700.058.41711414
ATTCGAG243800.058.28336313
AGTCCAT163250.057.89900218
Produced by FastQC (version 0.11.5)