FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18868357
Sequences flagged as poor quality0
Sequence length35-71
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA777150.062.6837417
GAGTCCG429900.062.4554917
AATGGAG779100.062.16109513
AGTCCGA226500.062.02528418
ATAGGAG490050.061.75067513
AGTCCAG587950.061.73639718
GGAGTCC1676950.061.5702716
AATCGAG271650.061.42712413
AGAGTCC410550.061.33294716
AAAGGAG754300.061.32158713
GAGTCCT1452150.061.21324517
AAACGAG305150.061.03986713
ATTGGAG789750.061.00461613
AGGAGTC662550.060.7245915
TCGAGTC299300.060.5106815
CGAGTCC530000.060.4770816
ATGGAGT479500.060.41320814
ATTCGAG273050.060.39237613
TGAGTCC206050.060.37513416
TAATGGA241450.060.18165612
Produced by FastQC (version 0.11.5)