FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22468457
Sequences flagged as poor quality0
Sequence length35-71
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA776600.062.50873617
AATGGAG791700.062.1048513
GGAGTCC1727250.061.8299916
GAGTCCT1517200.061.70074517
ATAGGAG495850.061.44376813
ATTGGAG767800.061.42399613
AAAGGAG777600.061.35418713
AGAGTCC439500.061.2359516
GAGTCCG486250.061.05457317
AGTCCAG591100.060.90275618
AATCGAG294150.060.5991513
CGAGTCC573000.060.305116
TGGAGTC907200.060.19457215
ATGGAGT479150.059.9517314
TCGAGTC314700.059.90632215
AGTCCGA271850.059.8956318
TAATGGA249100.059.813912
ATTCGAG287600.059.7949413
AAACGAG326150.059.6615213
AGGAGTC690900.059.56769615
Produced by FastQC (version 0.11.5)