FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21481218
Sequences flagged as poor quality0
Sequence length35-71
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCA806600.062.59586317
AATGGAG813450.061.92045213
GGAGTCC1778350.061.73243316
AAAGGAG797450.061.49404513
ATAGGAG522850.061.36232813
GAGTCCT1554450.061.17025817
GAGTCCG470750.061.1667517
AGAGTCC433200.060.99355716
ATTGGAG844750.060.9154113
AATCGAG296500.060.8683213
AGTCCAG608400.060.7443618
TCGAGTC322600.060.42915315
AGGAGTC708900.060.4172315
AAACGAG324900.060.2386913
CGAGTCC573950.060.13322416
ATGGAGT500150.059.99715814
TGGAGTC964150.059.8892515
ATTCGAG305950.059.73600813
TAATGGA288000.059.7326512
AGTCCGA265000.059.08410618
Produced by FastQC (version 0.11.5)