FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20010083
Sequences flagged as poor quality0
Sequence length35-71
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCCG403450.062.4452117
GAGTCCA696350.062.20820217
GGAGTCC1460700.061.8743216
AGTCCGA215300.061.6509318
ATAGGAG448250.061.48725513
ATTGGAG666950.061.45361713
GAGTCCT1326000.061.4451217
AAAGGAG693750.061.35978313
AATCGAG259200.061.2396813
AATGGAG671450.061.209713
AGAGTCC412550.060.87961616
AGTCCAG521400.060.5661718
ATTCGAG259950.060.14587413
AGGAGTC610900.060.10298515
TGAGTCC194500.060.04337716
CGAGTCC510950.059.9056216
AAACGAG293300.059.67782613
AAGGAGT402050.059.63190514
TCGAGTC286200.059.52472715
TGGAGTC775500.059.5014815
Produced by FastQC (version 0.11.5)