FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20850196
Sequences flagged as poor quality0
Sequence length35-231
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACAGAAGTACGGGAAGGCGAAGAAAAGAATAGAGAAGATAGGGAAAT8274963.9687684470687947No Hit
TGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTGAAGTGGAAAACTG5753972.75967189948718No Hit
GGGAGTTTCGTACTGAGGTGTAAAGGGATTTATATGGGGACGTAGGCCGA5395962.5879660795514825No Hit
CTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATG5048072.4211139310153245No Hit
GGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAA4867332.334428894577298No Hit
GGGTGTTGTAGGTTTCTCTTTTTCAGGCTTATACTCATGAATCTTGTCTG4003061.9199148055970314No Hit
GCGAACACTCTTTAATGGACCAGATCAGGATTTGAGCGGAAGAACGAATG3842891.8430953838515476No Hit
TAGAAGTTTGAAGTGGAAAACTGGAAGACAGAAGTACGGGAAGGCGAAGA3826551.8352585270661244No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT3676871.7634702330855785No Hit
GAAGGAGCGAGTGCAATTTGGTGATGAAGGTAGCAGGCGGCTTGGCTTGG3483581.6707660685779644No Hit
GTGATTGAAGCTGAGTACATTTTGCTGGTGTATTTTTAGGTAAAATGCTT3347211.60536140763377No Hit
AGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTT3332311.598215191838005No Hit
CCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAGTGTTGGGGCAATC3309861.5874479069645195No Hit
GGACTGAAGCCTTTAGTCTTTTCCAGATGCAACCTTAAAATCAGTGACAA2861431.37237558821989No Hit
GCGGCTTGGCTTGGCAACCACACGGAGGAGGCGAGCAGGCGTTGTGCGTA2812801.3490520664649868No Hit
GGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGT2793601.3398435199362155No Hit
GGTGATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTT2684901.2877097174530159No Hit
GCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTGAGTACATTTTGCTGG2588131.241297683724412No Hit
GATCCTGAAGGGATTTCTTCTGATGGTAGCTTTTGTATTATCAAGTAAGA2523621.2103579266113373No Hit
CTGCAGAGCAAAGGAAGTGGCTTAATGATCCTGAAGGGATTTCTTCTGAT2415831.1586605708646576No Hit
GAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAG2374361.1387710695861084No Hit
CAGAGGCATTTCATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATAT2361201.1324593783195132No Hit
GCAGGGAGAATTGCGTCATTTAAAGCCTAGTTAACGCATTTACTAAACGC2143471.0280335014596507No Hit
GTTGAGGAAATTTCTGCAGTTTTAAGCAGTCGTATTTGTGATTGAAGCTG1973240.9463891850225293No Hit
TTTAGGTAAAATGCTTTTTGTTCATTTCTGGTGGTGGGAGGGGACTGAAG1927900.9246435860842747No Hit
CCCTCACCTCGATGCAGCCAGTAGCTTGGATCCTTGTGGGCATGATCCAT1778470.8529751950533224No Hit
TCAGAAACTTTGTCTGCGAACACTCTTTAATGGACCAGATCAGGATTTGA1600980.7678488969600094No Hit
CATGGAGCCTTCCTGTGGCAGGAGAGACAACAAAGCGCTATTATCCTAAG1550470.7436237050241639No Hit
CTCTCCCCTCCCTTGGTCTTAATTCTTACATGCAGGAACACTCAGCAGAC1498670.7187798138684164No Hit
GTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCTCTCCCCTCCCTTG1451330.6960749913334149No Hit
AGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACG1451200.6960126417996262No Hit
CTAACTTCACAGAGAATGCAGTTGTCTTGACTTCAGGTCTGTCTGTTCTG1427140.6844731819307598No Hit
CTGTGCTGTGTGCCAATGTTTCGTTTGCCTCAGACAGGTATCTCTTCGTT1314890.6306367575633341No Hit
GCATGATGTGCTGTTAGAATCAGATGTTACTGCTAAAATTTACATGTTGT1300410.6236919787228858No Hit
GAAAATATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGG1246230.5977066114870094No Hit
CCACCCCGTGCCTTTTGATCTAGCACAGACCCTTCACCCCTCACCTCGAT1125580.5398414480132465No Hit
TCAAGAGTAATTACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGA1084460.5201198108641281No Hit
GATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGGAGTATGATTAAA1074240.5152181782847509No Hit
GGCTTAAAGTAGGACAACCATGGAGCCTTCCTGTGGCAGGAGAGACAACA1057080.5069880398246616No Hit
ATCCTTCATGAAGCCATTCAGGATTTTGAATTGCATATGAGTGCTTGGCT1038480.49806726037491444No Hit
AGAGATGAGTTGGGATCAAGTGGATTGAGGAGGCTGTGCTGTGTGCCAAT933090.447520972944331No Hit
CCAGTTGAATTCACCAGTGGACAAAATGAGGAAAACAGGTGAACAAGCTT792040.3798717287837486No Hit
ACCAACTTAATGTTTTTGCATTGGACTTTGAGTTAAGATTATTTTTTAAA616370.2956183241634755No Hit
CAGTACTGTTCTGATCCCGCTGCTATTAGAATGCATTGTGAAACGACTGG610510.29280779902500675No Hit
GAAGCCGAAATAAATGAGAGATGAGTTGGGATCAAGTGGATTGAGGAGGC590380.28315321352374817No Hit
GGGAACCACTAGTTCTTTCAGATGGTATTCTTCAGACTATAGAAGGAGCT526070.25230937877034826No Hit
CTCTGAAGGCTCTATGAAAGGAATAGCATGATGTGCTGTTAGAATCAGAT506070.24271714280287823No Hit
ATTCTTCAGACTATAGAAGGAGCTTCCAGTTGAATTCACCAGTGGACAAA351880.1687657996116679No Hit
TAGAGCCTTCTTTCCGCCAGGCCTTTCCCAATACCAACCGCTGGTTCCTC268260.12866066103167567No Hit
CTGCATTAACCAGCCCCAGTTCCGGGCTGTCTTGGGCGAAGTGAAACTGT254650.12213314445581232No Hit
TAGAAAGCTGTCTCCTTATTTAAATAAAATAGTGTTTGTCTGTAGTTCAG226740.10874717916320786No Hit
CACCATTGGCTCCTGTTTGAAATGAGCAGGCACTCCTTGGAGCAAAAGCC216490.10383115822987948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGT23900.0299.46518225
ATAACAT44600.0220.65413225
GGTCTAT11400.0166.76631225
GTAAGTG22500.0140.54063210-214
TATCCAG54950.0120.13339180-184
AGTGACT45350.0111.22541185-189
TTATAAT56000.0109.29785170-174
TAATACT56100.0107.73947175-179
GATAGTT17300.0107.28629210-214
GGGAGTT1044250.0103.685461
GGCATTT317700.0103.169945
CGTACTG1054100.0102.68479
AGTTTCG1057650.0102.6271444
TCGTACT1056100.0102.530878
TTCGTAC1060150.0102.2815557
CGAGTGC757950.0102.178278
GAGGCAT321450.0102.1665953
TTTCGTA1061750.0102.054046
AGGCATT323400.0101.417894
CTCTATT52500.0101.22039165-169
Produced by FastQC (version 0.11.5)