FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24006182
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1497510.6238018190481102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAC3150.032.735461
AAACGCG7500.021.87534914-15
AGGCGTA3800.019.7352548
GCGCTAG3004.3964974E-918.7484913
GTCGGTT26950.018.4354361
GCGGTTA4750.017.7617261
CGAAATC66000.017.0440832
TACGGTA7950.016.82625644-45
TATCGGT7500.016.36275760-61
CGTCAAA35650.016.3030364
GTCGGAA143750.016.1726111
CCGATAT17850.015.58673258-59
GTTACGA7350.015.3048911
CTTCGTT12600.015.2517481
GTTCGTA5257.2759576E-1215.177351
TACCGGG6650.015.17026242-43
CTATTAA48650.015.1259664
ATTTACG8700.015.0849926
CTCGTAA13400.015.0407671
TTTACGA9400.014.9589037
Produced by FastQC (version 0.11.5)