FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23685398
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGTTTACATTATGGCTAAATCTTTCAGTCTCAAGACTCAGCCAAGGT372100.1571010121932509No Hit
GGCAAAGTTTCGTGGATGCGGCAAGGGTTGCGGACCGCTGGCTGGGGGAT307640.12988593225243672No Hit
GCTCGTTCCTCCTCTGGCGCTCCAAGACGTTGTGTGTTCGCCTCTTGACA301630.12734850391789912No Hit
GGGAAGACCACCGAGGGGTCGATGCACTCTGAGGCGGCGGCGCTCAGATC281990.11905647521734698No Hit
GTAATTCCAGCGAGAGGCAGAGGGAGCGAGCGGGCGGCCGGCTAGGGTGG279330.11793342041370805No Hit
GGCTGGTGCATTTTCGGTTGTTGCTGATCTGTCTCAGGACTCTGACACTG273210.11534954996323052No Hit
GCAGTTTACATTATGGCTAAATCTTTCAGTCTCAAGACTCAGCCAAGGTT245230.10353636447232172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTA148200.025.861343
TACATTA152250.024.1514328
CTCGCGT1401.88725E-523.5734831
GTAATTC122700.022.8626291
TCGGTTC24200.021.606492
TTACATT175250.021.1966937
TCGCGTA6100.020.8502142
AGTTTAC182750.020.636074
CGCGTCT3503.6379788E-1220.2058451
CATACGT35100.019.717849
TGGCTAA168550.019.69276814-15
GTCGGAA121200.019.6834721
GCTAAAT168150.018.9696316-17
GTCGCGT89400.018.563461
TCGGAAA48150.018.4902232
GATTGCT219650.018.1840594-95
GGCTAAA161750.018.10474816-17
TAATTCC152600.017.9657362
ATTATGG181200.017.88930710-11
TTTACAT208150.017.8011256
Produced by FastQC (version 0.11.5)