FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23300012
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1720890.7385790187575869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA7600.030.9830272
CGCGTAA8250.027.4002633
CAGTTTA91900.024.3926433
TACATTA91600.023.1357988
AGGTATA36100.021.3945964
CCGATAT14250.021.36747658-59
GTCGCGT56050.020.7534221
TTACATT105750.020.5299557
GTCGGAA101700.019.680511
AAACGCG13250.019.55060814-15
TCGGTTC30000.019.4656032
GTAATTC96400.019.345791
TAGCATA18450.019.1439848
GGTATAA49450.019.04725
TATGTTA20050.019.0255875
TATACGA6950.018.9732047
ATGCGTT14400.018.6414556
CGGTTGC112100.018.610823
GTCGTCT41400.018.541961
CAGGTAT41750.018.4992793
Produced by FastQC (version 0.11.5)