FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23300012
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG3150.025.420763
TCGCGTA6600.024.9901642
TATAGGG64000.024.1235648-49
CAGTTTA92250.023.8962423
ATGCGTT13200.023.193056
ATATAGG19150.022.27532846-47
TACATTA91950.021.9747528
GTTCGTA8200.021.858041
GTCGGAA100550.021.4375721
TCGGTTC28900.021.0347252
GTCGGTT35850.020.1300011
CCGATAT13100.019.82158-59
TATACGA6800.019.3940097
AAACGCG14500.019.16471914-15
CGGTTGC116050.019.1172713
TTACATT107750.019.0584537
GTAATTC97050.019.0516241
GTCGCGT53050.018.8491571
AGGTATA38950.018.5015074
TCGGAAA42400.018.2273272
Produced by FastQC (version 0.11.5)