FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24311581
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGTTTACATTATGGCTAAATCTTTCAGTCTCAAGACTCAGCCAAGGT258900.10649245723673832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA5050.028.8918532
CAGTTTA109600.025.6664663
TACATTA112050.023.7604228
TATAGGG67550.023.35612548-49
CGCGTAA6250.022.5796243
GTCGGAA111000.022.2366891
CGCCGAT8750.021.5334591
GCGCTAG3351.8189894E-1221.0630823
TCGGAAA49650.021.0445082
GTAATTC114650.020.9535661
TTACATT129600.020.7243377
CATACGT36350.019.9280999
ATATAGG24650.019.38845846-47
GTCGCGT65450.018.7841991
GGCTAAA123450.018.59419416-17
TCGGTTC28700.018.3671262
GGGGGGA374400.018.2782082
CGGTTGC110900.017.9425543
GGTATAA63600.017.8981725
AGTTTAC151850.017.8116954
Produced by FastQC (version 0.11.5)