FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23114290
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN800210.34619709279411137No Hit
GGCAGTTTACATTATGGCTAAATCTTTCAGTCTCAAGACTCAGCCAAGGT368540.1594424920687592No Hit
GCTCGTTCCTCCTCTGGCGCTCCAAGACGTTGTGTGTTCGCCTCTTGACA294830.12755312838940758No Hit
GGGAAGACCACCGAGGGGTCGATGCACTCTGAGGCGGCGGCGCTCAGATC290610.12572741797390274No Hit
GGCAAAGTTTCGTGGATGCGGCAAGGGTTGCGGACCGCTGGCTGGGGGAT285850.1236680858464612No Hit
GCAGTTTACATTATGGCTAAATCTTTCAGTCTCAAGACTCAGCCAAGGTT269520.1166031922243772No Hit
GTAATTCCAGCGAGAGGCAGAGGGAGCGAGCGGGCGGCCGGCTAGGGTGG267940.11591963240056259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTA145500.025.6018433
CGCCGAT4050.025.5816331
GTAATTC117900.023.7264751
TCGCGTA5650.023.33822
TACATTA156700.022.9605438
AGTTTAC170600.021.3382234
GCTCGCG40150.020.4089876
TTACATT176950.020.3595737
CGCGTAA7400.019.7282123
GTTCGCG2750.019.70774744-45
TAATTCC138700.019.455212
TGGCTAA165700.019.35634614-15
GCTAAAT173400.018.48852516-17
ATTATGG177450.018.3648710-11
ATTTACG5450.018.1459856
ACATTAT204600.017.631169
AAACGCG13200.017.48319814-15
GCAGTTT223550.017.4426882
GTCGGAA100050.017.3217331
CAACGTA58650.017.183184
Produced by FastQC (version 0.11.5)