FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26239318
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAGTTTCGTGGATGCGGCAAGGGTTGCGGACCGCTGGCTGGGGGAT404480.15415034796254992No Hit
GGCAGTTTACATTATGGCTAAATCTTTCAGTCTCAAGACTCAGCCAAGGT392210.14947415935124533No Hit
GCTCGTTCCTCCTCTGGCGCTCCAAGACGTTGTGTGTTCGCCTCTTGACA357410.13621161952456232No Hit
GTAATTCCAGCGAGAGGCAGAGGGAGCGAGCGGGCGGCCGGCTAGGGTGG327060.12464500792284312No Hit
GGCTGGTGCATTTTCGGTTGTTGCTGATCTGTCTCAGGACTCTGACACTG321050.12235455205047631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTTA151050.026.5204643
TACATTA161250.024.3461538
GTAATTC146750.023.744891
CTCGCGT1201.832881E-423.5125681
TCGCGTA6750.022.2746942
AGTTTAC180150.021.9746974
TTACATT181400.021.7970947
TCGGTTC21700.021.6523882
TAATTCC158400.021.4757792
TGGCTAA173950.019.53152814-15
TATAGGG62050.019.43826148-49
CGCCAAT4150.019.2633081
ATGCGTT11850.019.0215856
GGCTAAA167400.019.00539216-17
TGATTGC198700.018.95859194-95
GCTAAAT176400.018.887616-17
GTCGATA7900.018.45291
GCAGTTT230500.017.9992892
ACATTAT221300.017.8246789
ATTATGG190950.017.73121810-11
Produced by FastQC (version 0.11.5)