FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22331104
Sequences flagged as poor quality0
Sequence length35-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG304230.13623598725795194No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT286430.12826504233736047No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT278050.12451242894216066No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG226430.10139668867244539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAG315500.031.1878575
GGGGAGA359450.030.7158324
TAGGGGG207400.030.61036350-51
TAGCATA101500.030.2660589
TTAGGGG145300.030.0852548-49
GAATAAA183950.029.7244966-67
AAAAAGG791650.028.80132578-79
AAGTTTT341150.028.65968728-29
AGTTTTT343600.028.56765730-31
TGTTACG13400.028.3881283
AGAGGGG282300.027.730178
GAGGGGG464650.026.6454969
TTTAGGG172850.026.38727448-49
TAAGGGG155450.026.36205750-51
TTAAGGG129800.025.88523548-49
AAAGGGG873850.025.88126280-81
AAAAGTT378800.025.5070426-27
TTTTAGG172000.025.21950146-47
ATAAAAA430700.025.12226568-69
CCGTCGG88550.024.5789742
Produced by FastQC (version 0.11.5)