FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19307656
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2988541.547852313092796No Hit
GCTCGGTTGCCCATAGGTGTTCTGCTGAGAGTAACTGCTCTGATCATAAC308520.15979153554424214No Hit
CTCGGTTGCCCATAGGTGTTCTGCTGAGAGTAACTGCTCTGATCATAACT276840.14338353656186956No Hit
GAGAAAATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCA238310.12342772214296753No Hit
GGTGACTGTAGCAGTGGTGGTATCATAAGCACCAGTGCCATACCCCTGGA226530.11732651545065854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG7750.027.2345522
CTCGCGT1301.1034472E-525.2646831
GCTCGCG10450.024.2357886
GCGCTTA1551.5767037E-624.208492
GCGCTAG3700.024.0830863
CCGTACG20650.020.8952454
GCGCATT16200.020.5637571
AGTACGC23200.020.47428148-49
GTTCGTA9050.020.2197191
TCGCGTA9400.019.9591262
TCCGTAC21950.019.6568343
TATCGGT9150.019.61155760-61
TTAGTCG1452.6866473E-919.50681156-57
GGCTAAA68400.019.36757316-17
GCCTAAT9000.019.2893831
AGCGAAG22600.018.8848116
CTCCGTA18950.018.8110832
CGCGTAA9750.018.7594593
CAGTTTA86650.018.5104853
TAGTCGG160100.017.9562450-51
Produced by FastQC (version 0.11.5)