FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481826
Sequences flagged as poor quality0
Sequence length35-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG535010.2894789724781523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT3100.023.500551
AGAGTCG27150.022.1159325
GAGTCGC28650.021.7539566
CCGTATC7200.020.8548012
AGTCGCC28650.020.6437827
GTATCGT4900.020.4226134
GTTCGGA12300.019.07485832-33
CCTAGTC17800.017.93822392-93
CGCCAAT4650.017.6254121
TTTAGGG76100.017.58692448-49
CGCAGTA48100.017.4949913
AAAGGGG468050.017.39553880-81
GCGCTAG4601.8189894E-1216.8104043
CGTTTTA23750.016.2795493
TAGGGGG107250.016.22213750-51
CCCGTAT7200.015.8098331
GTCGTAA13500.015.5147151
CGGCTAT6750.014.8401621
CGCAATT16900.014.8034533
GTCGCCG40950.014.7774668
Produced by FastQC (version 0.11.5)