FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19228173
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193980.10088321963818403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT2600.028.5312121
AAAGGGG427850.018.56947580-81
CGCCAAT4300.018.3296451
TAGGGGG101750.018.14208250-51
AAAAAGG476700.017.03944678-79
GGGAAAA487700.016.68857222-23
GAATAAA118400.015.83331366-67
GTATCGT7950.015.7285954
GAGTCGC25150.014.9171776
TTAGGGG84650.014.7870748-49
GTCGACT11100.014.6190341
CGCCGAT5853.6379788E-1214.2656071
TTTTAAT209350.014.02054332-33
TTTAGGG95200.013.662559548-49
TAAGGGG117100.013.65144950-51
CGCCCTT18200.013.5013771
GTTGGGA154350.013.47877818-19
GGGGAGA273950.013.4058754
AAAGTTT223900.013.39396128-29
GTTCGGA10700.013.217525532-33
Produced by FastQC (version 0.11.5)