FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23700531
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG625470.2639054795860903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT7900.031.8419781
CTCGCGT4650.024.5896021
CGCGTTT5400.020.3274041
CGTTTAG8150.019.0803241
TTAGGGG97700.017.8704948-49
CGCGAAT1801.649493E-417.7864781
AAAGGGG636300.016.5763680-81
CGCCGAT7200.015.8807841
AAAAAGG657950.015.75144678-79
GGGAAAA677400.015.54466522-23
TAGGGGG165100.015.520802550-51
GAGTCGC38750.015.4481066
GAATAAA142450.015.0081266-67
GGAATAA138450.014.07584264-65
CGCCCTT18350.013.9577821
TTTAGGG136450.013.79277848-49
GGTTTTT450550.013.44393842-43
CGCGACT4153.2074786E-713.2250591
AGAGTCG42000.013.1636685
GTCCGGT26450.013.1417241
Produced by FastQC (version 0.11.5)