FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17929736
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG472600.021.80162278-79
AAAGGGG476100.021.40826680-81
GAATAAA105700.021.39012566-67
GGGAAAA520400.020.15472822-23
GGGTTTT280050.019.15674242-43
AGAGGGG188550.018.843018
GGGGGAG358750.017.6835443
TCGCGTA8550.017.4525552
AAAAGGG455050.017.35002978-79
GGGGAGA282450.017.1464654
AAAGTTT223900.016.91755728-29
GTCGCGA1952.6093388E-416.7502841
GTATCGT9100.016.3911444
ATTCGCC20800.016.36684416-17
ATTTACG7450.016.269296
AAAAGTT241850.016.00738726-27
TAGGGGG160250.015.92479850-51
CGCCCTT11450.015.8934411
GGGGTTT325400.015.86279640-41
GGGGAAT193750.015.74684862-63
Produced by FastQC (version 0.11.5)