FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15449434
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5142453.3285685417342794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT3650.021.4064661
GCGCTAG4650.020.7464773
TTCGTCT26800.020.5695363
CGCGTAT4000.019.5239183
TCGCGTA6600.018.7952162
ATAGCGC1252.881101E-617.9468682-83
CGTAGGG8400.017.17404276-77
GTCGTAA23600.016.7484781
GCGATAT2758.3955274E-716.7049563
CGCCCTT10000.016.0863271
GCGTAGA2154.996764E-915.95538992-93
GAGTGTA50600.015.6167055
GCTTCGT37900.015.279891
TCGCTAA21250.015.05819258-59
TGCGCTA8650.014.8722
CGTAATA21950.014.859423
CGTATAA3100.014.69118568-69
GGCGTAG15500.014.58295194-95
ATTCGCC12000.014.54582116-17
GTCCGAT4755.020411E-1014.513981
Produced by FastQC (version 0.11.5)