FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11581541
Sequences flagged as poor quality0
Sequence length35-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3725753.2169725945795986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG451.7880666E-976.7854662-63
TACGTCG750.059.177712
CGATTTA359.000452E-657.6401146
GGATCGG39500.045.178294-95
ACGCGAA1302.2690438E-741.55239590-91
GTTAGCG250.001457178538.81075760-61
CGCTAAC1300.037.2447133
CTCGCGT3150.037.155961
CGATACG1550.036.4374129
CCATACG1003.1523086E-936.3135954
CGGATCG9150.035.14082794-95
GTTCGAC1450.035.066886-87
GGGATCG40950.034.5487194-95
ATACGCT851.307335E-633.2278336
TACGGTG1351.7716957E-929.8956011
AACGCGA554.4494023E-429.74056280-81
GCGCTAA1651.6370905E-1129.344322
GTACCGG1605.629162E-428.56882794-95
TTAGCGC805.491651E-627.64007678-79
CGTCGAC2350.027.478511
Produced by FastQC (version 0.11.5)