FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11581541
Sequences flagged as poor quality0
Sequence length35-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG635420.548648923316854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT901.2189066E-648.920690-91
ACGCGTT1951.03682396E-1038.94117492-93
CGTCGAC1700.033.647771
CGCGCTA705.031058E-429.1229259
CGCGATA854.5709272E-528.8409441
CGCGTCT1300.00917880826.65007694-95
CCTAACG1609.738869E-925.482457
CGCCTAA1451.05737854E-725.3079095
ACGTTCG1001.3946333E-424.479113
CGTACCG1001.4008193E-424.4631527
CGCCGAT3750.023.970031
CGCGTTA1201.73337E-523.8338361
CTAACGC3250.023.20938552-53
ATACGCG2602.8893137E-622.57873790-91
GCGTATC1102.6719732E-422.2393269
GGGGCTA44200.021.8155881
TACCTAG22450.021.2487099
CGAATCG957.3726005E-720.69179550-51
GTTAGCG956.8241097E-620.44736160-61
ACCGATC4800.020.385966
Produced by FastQC (version 0.11.5)