FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22767296
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1630490.7161544348525183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT4300.028.9710311
GTATCGT4050.025.219724
CGCGAAT1752.8002432E-722.8812031
CTAGCGA3008.38736E-917.7964921
CCGTATC9850.017.6024882
CGTCGGT2807.392373E-817.4786971
CGAGGTA21950.016.0006732
ATGCGTT16950.015.7156596
TCGCGTA4251.546141E-1015.69090752
CGCCAAT7600.015.2206831
CGTACTA2903.4854354E-513.7971762
CGGGGGT153550.013.5023851
CCCGTAT12200.013.4932411
CGTATCG8350.013.3021653
CGCGATA2702.9235E-413.1825861
GTCGGTA22050.013.1153281
GTCGACT6051.7826096E-1012.5016671
CGCTCAA12250.011.9631434
GTCCGAT7503.6379788E-1211.8643271
CCCGTTT45800.011.7542331
Produced by FastQC (version 0.11.5)