FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28207408
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN452350.16036567415198164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG600.004365619331.0299342
CTCGCGT1201.9617268E-423.278031
CGCCAAT1754.825255E-621.2827721
CGGTCTA15650.021.1160684
GTCGGTT38650.020.5978981
TACGTCG704.7260238E-420.4390370-71
TACGCAA24200.020.3833839
CGTCGGT2151.3099179E-619.4885861
TTTACGC28150.018.845637
TCGCGTA5950.017.9921472
GTCGCGA8900.017.262361
CGTTCGA5350.016.526627
CGAGGTA30700.016.222492
CCCGTAT5650.015.6560221
CGCGTCT3958.538336E-915.3222491
ATCCGTT41750.015.1655691
TTAGTCG2201.9808795E-914.9326356-57
CCGTTCG4950.014.66991454-55
CGTAATA36400.014.5771473
GTATCGT4855.675247E-1014.3952284
Produced by FastQC (version 0.11.5)