FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22747602
Sequences flagged as poor quality0
Sequence length35-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1000770.4399452742315432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTA79100.025.5774063
CGCCAAT2051.2205419E-924.9627171
CGCGTCT2053.335481E-822.6933781
TACGTCG5500.022.55514570-71
GTCCGAT4000.022.097681
CGTCGAC12750.019.7031941
CTATTAA102350.019.5854324
GCGCTAG4250.018.6038883
GCGTATA7750.018.6038884
CGCCCTT11350.018.444621
GTCGGTT27300.018.4040831
CGAAATC119800.018.1691912
GCGTTAA4650.018.0002258
CGCTCAA18650.017.7061124
GTCGCGA4500.017.5747621
TCGATCC41750.017.31433194-95
CGTCAAA65150.017.2760564
CTCGGTA84500.016.8426842
GTATTCG81300.016.7617666
TCCGAAA93700.016.7277262
Produced by FastQC (version 0.11.5)