FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26327109
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN626540.2379828335879948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG4200.030.0316453
CGTTCGA5200.021.557327
TACGTCG2050.020.88358170-71
GCGTATA34300.019.067714
TTAGTCG1757.4578566E-1118.80099756-57
GTCGGTT26250.018.513221
GGCGTAT36150.018.2211363
GTCGTAA35100.017.7060811
CCGTTCG4500.017.21205354-55
GCGCATT36150.017.0581822
TCGTAAT35900.016.6564562
GTCCGAT5400.016.441361
TCGCGTA6850.016.3677062
GCGCTTA1750.00242245816.0210551
CGTAATA31000.015.6724273
TATTCGG45750.015.6202227
CTCGTTT50050.015.4982231
CGTATAG43150.015.3734215
GTCACGT56700.015.2463741
TCGATAG2155.438352E-415.2098243
Produced by FastQC (version 0.11.5)