FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29427155
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN495950.16853481078955815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT3450.022.7275894
GCGCTAT1755.219368E-621.0907271
CCGTATC17700.018.5017282
CGTATTC8250.018.4494553
GCCGTAT19850.016.9668791
CGCCGAT19550.016.2832811
CACCGGT5400.016.2330251
GCGCTAG7600.015.7791393
TCGCGTA5300.015.6647652
GTCCGAT9050.015.2936911
GGATCGG75050.015.1078294-95
CGCGTAT7050.015.0474283
CTCGTTT94200.014.7909361
GCGATAT8950.014.4297413
CCCGTAT17650.014.3766461
GGGATCG64750.014.31787694-95
AACGCGC18300.014.23778814-15
CTCGCGT1950.00541759914.1956821
GAGTGTA93600.014.1917685
TCCGATT2952.8952736E-514.0717382
Produced by FastQC (version 0.11.5)