FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004976706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350096261
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGG8246785723.555766281091472No Hit
GTCCGGTC7367077021.043003941136064No Hit
AAGATCAT6009024817.163921667818098No Hit
TGATCTCA4227657012.075698803307128No Hit
TGATCCCA117609753.359354643321941No Hit
CCAGAAGG98230292.80580802889523No Hit
CCCGAAGG68543831.957856670740051No Hit
GTCCGGCC65223381.863012755797469No Hit
GTCCGGGC62651441.7895489606499968No Hit
CCGGAAGG61063911.7442034320954944No Hit
AAGATCAG48577041.3875338131646027No Hit
AAGAACAT46063031.3157247057831332No Hit
AAGATCAA43955891.255537259222543No Hit
GTCCGGAC38555821.101291967239833No Hit
TGAACTCA26783260.7650255939180111No Hit
AAGATCAC26565980.7588193008436614No Hit
GACCGGTC26164280.7473453136935958No Hit
TGAGCTCA25102140.7170068005953368No Hit
TGATCGCA24211180.691557799870362No Hit
AAGAGCAT20853610.5956536051094816No Hit
GCCCGGTC15920210.45473807559458623No Hit
GGATCTCA15045690.4297586600046551No Hit
AAGACCAT12115320.3460568234974666No Hit
GGCCGGTC10202690.29142527746104663No Hit
CGATCTCA9540350.2725064807247399No Hit
TGACCTCA8468560.24189232915001058No Hit
AGATCTCA8222300.2348582637390692No Hit
TGATCACA7375690.21067605746295015No Hit
NCTGAAGG5441510.15542896643503426No Hit
NTCCGGTC4277620.122184109815443No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)