FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004976707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250974825
Sequences flagged as poor quality0
Sequence length8
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC7565910530.146093338246178No Hit
GAGGATCT3783951115.07701459698199No Hit
CTTATGAG3420019513.626942463253036No Hit
AGACCATA3396519913.533309167562921No Hit
AGACCAAA68110962.7138562602842735No Hit
GAGGATCC59171962.35768507857312No Hit
AGACCACA56901442.2672170405936134No Hit
TCCGGCGC46268061.8435339082316322No Hit
GAGGAACT42651891.69944893875312No Hit
AGACCAGA39400891.569914034206419No Hit
ACCTGCGC37058331.4765755888065666No Hit
CCCTGCGC36904331.470439515198387No Hit
TCCCGCGC33669331.3415421247927954No Hit
GCCTGCGC32017291.2757171959378795No Hit
TCCAGCGC28107781.1199442015747993No Hit
CGTATGAG28084041.118998289967928No Hit
GAGGAGCT27800001.1076808201778805No Hit
GAGGATCG27088971.079350090193309No Hit
GAGGATCA20864340.8313319871823797No Hit
GAGGACCT14544290.579511909212408No Hit
CTCATGAG12426780.4951404986536No Hit
CCTATGAG12248260.48802743462417No Hit
CTGATGAG12232310.4873919127147514No Hit
CATATGAG9658890.3848549351513643No Hit
CTAATGAG8379530.33387930442824293No Hit
CTTAGGAG6766980.26962784016285296No Hit
CTTACGAG6130550.2442695198612052No Hit
NCCTGCGC5785400.23051714449845717No Hit
CTTAAGAG4929260.1964045597003604No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)