FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120336815
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCACC2831735423.531746290609405No Hit
ACGGACAT2605446421.651282693496583No Hit
TTCAGGTG1504320912.500920021856984No Hit
GATCTTGA1476355512.268527299812613No Hit
ACGGACAG52986894.403215258771807No Hit
CGAACACC47229923.924810541146531No Hit
ACGGACAA47039833.909014045286141No Hit
CGAGCACC41284783.430768879831164No Hit
ACGGACAC38386033.189882497721084No Hit
CGACCACC33429502.777994415092339Illumina Single End PCR Primer 1 (100% over 8bp)
TTCAGGGG14045961.167220521832824No Hit
GAGCTTGA12657231.0518169356568063No Hit
GTCAGGTG6971270.5793131553298964No Hit
TGCAGGTG6930980.5759650527562991No Hit
GAACTTGA6659410.5533975616688874No Hit
NGATCACC5522430.4589144228223092No Hit
GACCTTGA4805890.39936988526744704No Hit
NCGGACAT4664390.38761122271683857No Hit
TTCAGGCG4299820.3573154233806171No Hit
ATCAGGTG3838660.3189929864771641No Hit
TTCAGGAG2996720.24902769779971326No Hit
GATCTGGA2895410.2406088278138324No Hit
TCCAGGTG2806700.23323701894553214No Hit
GATCGTGA2783610.23131823789752123Illumina PCR Primer Index 1 (100% over 8bp)
GATCTCGA2656890.22078779465785262No Hit
GATCCTGA2525180.20984268197558661No Hit
CTCAGGTG2433110.20219165680926487No Hit
NTCAGGTG2336440.19415837123493754No Hit
NATCTTGA2095690.17415202488116377No Hit
GATCTAGA1673410.1390605194262454ABI Dynabead EcoP Oligo (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)