FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146559477
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAGGCC3470189923.677690252674687No Hit
GATGCCAA2842120519.3922669361054No Hit
CTCCTAGT2385121816.27408782306176No Hit
AGGTATTG1919365313.09615276533772No Hit
GCAAGGCC67354884.595736923924749No Hit
ACAAGGCC48611013.316811099155328No Hit
GAAGCCAA37230742.540316106613836No Hit
CCAAGGCC36395582.4833317329591726No Hit
GAGGCCAA36155582.466956128671229No Hit
GACGCCAA23939181.6334105777410766No Hit
AGGGATTG21598701.4737156847250485No Hit
CTCCTAGG19329601.3188911693509933No Hit
NCAAGGCC13828520.9435432141996523No Hit
AGGTAGTG10837500.739460881127462No Hit
CTCCGAGT9502630.6483804523947639No Hit
AGGTATGG9432480.643593999724767No Hit
CCCCTAGT7066060.48212917681195055No Hit
NATGCCAA6007550.4099052564168198No Hit
CTCCTAGA5974980.40768295045157676No Hit
CGCCTAGT5326730.3634517609529952No Hit
AGGAATTG4886000.3333800106287224No Hit
AGGTATCG4772620.32564390223635964No Hit
AGGCATTG4600130.31387461897124536No Hit
CTCCTAGC4384190.29914066901316794No Hit
CTCCCAGT3997490.2727554766042185No Hit
AGGTAATG3947430.2693397984764916No Hit
NTCCTAGT3159140.2155534438758948No Hit
CTCCAAGT2681340.18295234500598007No Hit
CACCTAGT2274240.15517522623255542No Hit
NGGTATTG2023510.13806749596957146No Hit
AGGTACTG1660180.11327687802816053No Hit
AGGTATAG1654150.11286544097042595No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)