FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004976721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339448488
Sequences flagged as poor quality0
Sequence length8
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTACGC8662264225.518641284977534No Hit
CCCAGGAT7311105321.53818785016948No Hit
GTTCCTCA4844375214.27131176380435ABI Solid3 Adapter A (100% over 8bp)
TAAGTATG316461759.322821022552324No Hit
CCCAGGAA150258844.426557940655785No Hit
CCCAGGAG115147043.3921800824165107No Hit
AGGAACGC114317763.3677498660709895No Hit
AGGGACGC114104913.361479400668298No Hit
CCCAGGAC99570652.9333066288396608No Hit
AGGCACGC71769932.1143099037754443No Hit
GTTCCCCA69074282.0348972654725745No Hit
GTTCCGCA27264070.8031872570898003No Hit
GGTCCTCA25912020.7633564713359395No Hit
GATCCTCA21175630.6238245491905093No Hit
GCTCCTCA18529270.5458639721500247No Hit
TAAGGATG17559470.5172940997162433No Hit
GTACCTCA14611330.43044321941419283No Hit
GAAGTATG14302790.4213537695887453No Hit
GTCCCTCA14193220.4181258865999132No Hit
TAAGTACG13600700.40067051351838695No Hit
TAAGTAAG12062330.355350824246417No Hit
TAAGTAGG8622380.2540114422309638No Hit
GTGCCTCA8504420.2505363936103318No Hit
TAAGAATG8297960.24445417473769981No Hit
CAAGTATG7486930.22056159519555732No Hit
TAAGCATG7197000.21202038761180164No Hit
GTTCCACA5934470.17482682085182832No Hit
AAAGTATG5369410.1581804070371938No Hit
NGGTACGC5302160.15619925224118247No Hit
NCCAGGAT4480950.13200677447118278No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)