FastQCFastQC Report
Sat 10 Sep 2022
EGAF00004976724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15594376
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCCTT393884825.258131521261255No Hit
CAGATGGG378796024.29055192718195No Hit
TCACATCA370860323.78166975068448Illumina PCR Primer Index 1 (100% over 8bp)
AGTGGAAC369065623.666583388780673No Hit
TCCCATCA363240.2329301281436333No Hit
AGTGGACC339960.2180016693197599No Hit
CAGCTGGG252860.16214820009470082No Hit
AGTGGCAC241690.1549853613892598No Hit
CAGATGGA178700.11459259415060917No Hit
GTCTCCTA171540.11000119530271682No Hit
TCACCTCA156370.1002733293079505RNA PCR Primer, Index 40 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)