FastQCFastQC Report
Fri 1 Jul 2022
EGAF00004976730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400459655
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGCTG9325499923.286989796762423No Hit
TCCAATGC8085132120.189629589527563No Hit
GTAGCACA6768871216.90275441105297No Hit
CGTTTGAT5212520813.016344430501995No Hit
AAGCGCCG195564854.883509426186765No Hit
AAGCGCAG121638593.037474274405995No Hit
AAGCGCGG104980092.621489797767518No Hit
GGAGCACA90931892.270687917363361No Hit
GAAGCACA69923261.7460750197170298No Hit
GCAGCACA62890131.5704485886349775No Hit
GCCAATGC53419541.3339556015948721No Hit
TCCAAAGC46083451.1507638640901292No Hit
ACCAATGC33797710.8439729090811907No Hit
TCCAACGC31375130.7834779261346564No Hit
CCCAATGC27868530.6959135496433467No Hit
CGTTTGAG25202240.6293328100679705No Hit
CGTGTGAT23273980.581181642380429No Hit
TCCAAGGC21334460.532749297803795No Hit
CGGTTGAT19414740.4848113850570041No Hit
CGTTCGAT17303900.432100956587No Hit
CGTTGGAT16773220.4188491846950225No Hit
CGATTGAT13535900.33800908108957944No Hit
CGTTTGAA12832650.32044801117356003No Hit
CGCTTGAT10279900.25670251351537526No Hit
CGTTTGAC9375800.23412595708299255No Hit
CGTCTGAT7039840.1757939885354993No Hit
GTACCACA6728710.16802466655473697No Hit
NCCAATGC5783900.14443152831463135No Hit
NAGCGCTG5740790.14335501537601833No Hit
CGTATGAT5628570.1405527355808165No Hit
CGTTAGAT5281110.13187620610620562No Hit
NTAGCACA4258860.10634929004271355No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)