FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31212215
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG383390.12283332022414942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177500.038.4886471
GTATCAA381150.036.4359861
CAACGCA481900.027.610815
ATCAACG490950.027.2317583
TCAACGC491350.027.1118644
AACGCAG505150.026.46426
TATCAAC513150.026.387752
ACGCAGA532550.025.0768437
CGCAGAG548650.024.3677278
AGAGTAC561200.023.7232311
AGTACGG422450.022.83257513
GAGTACG437050.022.14991412
CAGAGTA611450.021.97416110
GTACGGG447450.021.57223714
AGGTGTA181650.021.17772970
GCAGAGT647600.020.7356599
TACGGGG281400.020.6954415
TACGGGA121700.018.37619815
GAGGTGT267150.016.4308370
GAGTACT233050.015.63336912
Produced by FastQC (version 0.11.5)