FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32162912
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA229300.043.9551661
GTATCAA494650.037.643661
CAACGCA678750.026.7645455
TATCAAC693600.026.4642752
ATCAACG697200.026.2168883
TCAACGC696550.026.201194
AACGCAG715750.025.4592256
ACGCAGA749650.024.30957
CGCAGAG752600.024.1806818
AGAGTAC769200.023.61870211
AGTACGG548450.021.68379613
CAGAGTA841600.021.65708710
GAGTACG557750.021.33475512
GCAGAGT867200.021.1021219
GTACGGG577750.020.55989614
TACGGGG340900.019.7424615
GAGTACT340700.018.50077212
TACGGGA163200.018.31414815
TCGTATG34800.017.3001940
CGTATGC36450.016.70911241
Produced by FastQC (version 0.11.5)