FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32162912
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212750.042.6141431
GTATCAA452150.036.3772051
CAACGCA593950.026.9964245
ATCAACG610250.026.4675663
TCAACGC610550.026.4438534
TATCAAC613350.026.3058172
AACGCAG626200.025.874066
ACGCAGA657150.024.6769127
CGCAGAG660400.024.577588
AGAGTAC676800.023.76150311
AGTACGG533000.021.93777713
CAGAGTA746000.021.7965510
GAGTACG542850.021.63024312
GCAGAGT773700.021.032919
GTACGGG560800.020.78143514
TACGGGG326050.020.29775415
TACGGGA159200.017.74071315
GAGTACT256550.016.91602912
ACGGGGG152900.016.54880716
TACGGGT66600.015.39692715
Produced by FastQC (version 0.11.5)