FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32675699
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA189300.040.3833921
GTATCAA421050.037.6842651
ATCAACG568550.027.2789273
TCAACGC574050.026.9992334
CAACGCA581700.026.7103545
TATCAAC591900.026.3922312
AACGCAG603700.025.731186
ACGCAGA637100.024.3515827
CGCAGAG663650.023.355538
AGAGTAC665800.023.29179811
AGTACGG500350.021.94264413
CAGAGTA728500.021.39263210
GAGTACG527400.020.85697412
GTACGGG529100.020.72387514
TACGGGG376050.020.11210615
GCAGAGT784350.019.873069
TACGGGA137500.017.66465415
ACGGGGA204500.015.35119416
GAGTACT295400.015.30731712
ACGGGGG182100.014.97168116
Produced by FastQC (version 0.11.5)