FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29497991
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA178550.040.3433841
GTATCAA371450.034.9761051
ATCAACG491450.025.8400673
CAACGCA490050.025.8138895
TCAACGC493300.025.6509154
TATCAAC516450.024.6977842
AACGCAG517700.024.5433256
ACGCAGA550600.023.0417217
CGCAGAG569300.022.3212178
AGAGTAC578400.022.03158811
AGTACGG368700.020.959613
CAGAGTA635350.020.10043310
GTACGGG388700.019.83613414
TACGGGG286050.019.80897315
GAGTACG391150.019.7744912
GCAGAGT674400.018.9929289
AATCTCG51550.016.70305670
GAGTACT300200.016.66011212
ACGGGGA159450.015.86969816
TACGGGA100600.014.7859115
Produced by FastQC (version 0.11.5)