FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29497991
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164050.038.0779951
GTATCAA320950.032.2276421
CAACGCA398300.024.8680525
TCAACGC400550.024.7834174
ATCAACG401400.024.7435553
AACGCAG424250.023.4457976
TATCAAC428300.023.4320222
ACGCAGA451250.021.9890467
CGCAGAG468150.021.27848
AGAGTAC476650.020.83835411
AGTACGG344700.019.89027813
GAGTACG364350.018.85611312
CAGAGTA532200.018.79487610
GTACGGG365550.018.7269414
TACGGGG268600.018.34603115
GCAGAGT572900.017.562679
TACGGGA98350.014.69674615
CTGTGTA229000.014.27642570
GAGTACT217600.014.12167612
ACGGGGA154400.013.94012516
Produced by FastQC (version 0.11.5)