FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004976748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36493820
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA206750.037.17431
GTATCAA420000.034.5731161
CAACGCA537500.025.9460285
TCAACGC551800.025.4411034
ATCAACG552800.025.4374853
AACGCAG571300.024.4964456
TATCAAC578900.024.4958292
ACGCAGA606150.023.0710147
CGCAGAG612050.022.8497438
AGAGTAC627600.022.30076611
AGTACGG463400.021.35860613
GAGTACG478800.020.73019612
GTACGGG485800.020.3880414
CAGAGTA701850.020.06123410
TACGGGG322850.019.94635815
GCAGAGT743500.018.9360779
TACGGGA138900.015.72276515
ACGGGGA176750.015.02873316
GAGTACT277500.014.79402512
ACGGGGG168500.014.39372916
Produced by FastQC (version 0.11.5)