FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004976759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53686
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1540.2868531833252617No Hit
TCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1520.28312781730805053No Hit
ATATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1410.26263830421338896No Hit
ACATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1340.2495995231531498No Hit
TTCGGTTGATAAGCCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1220.22724732704988265No Hit
TAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1190.22165927802406588No Hit
AAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1170.2179339120068547No Hit
CAATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1060.19744439891219312No Hit
CTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA920.1713668367917148No Hit
CCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA900.1676414707745036No Hit
TGATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA850.15832805573147563No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA840.15646537272287003No Hit
TTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA820.15274000670565882No Hit
GTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA810.15087732369705323No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT750.13970122564541967No Hit
TTCGGTTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT750.13970122564541967No Hit
TTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT730.13597585962820846No Hit
GCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA730.13597585962820846No Hit
TTATCCTGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTTCCCTAACT730.13597585962820846No Hit
ATCGGTTGATAAGCCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA680.1266624445851805No Hit
GTATCCTGATGCACCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG680.1266624445851805No Hit
TTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT660.1229370785679693No Hit
TTATCCTGATAAGCCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA660.1229370785679693No Hit
TCATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG630.11734902954215252No Hit
TCCGGTTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT610.11362366352494133No Hit
GTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT600.11176098051633573No Hit
TTCGGTTGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT600.11176098051633573No Hit
GCATCCTGATGCACCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG590.10989829750773013No Hit
TTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA570.10617293149051893No Hit
CTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT570.10617293149051893No Hit
CTCGGTTGACAACTCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT560.10431024848191336No Hit
TGATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA560.10431024848191336No Hit
AGATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA560.10431024848191336No Hit
TGCGGTTGATAAGCCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA550.10244756547330776No Hit
TTATCCTGAGGAATCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC550.10244756547330776No Hit
CTCGGTTGAAGGAGCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT550.10244756547330776No Hit
TAATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG550.10244756547330776No Hit
CTATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA550.10244756547330776No Hit
CCATCCTGAAGGAGCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT540.10058488246470217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)