FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004976763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188234
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA3330.17690746623883039No Hit
AGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA3070.16309487127724004No Hit
TTTCACGGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3030.1609698566677646No Hit
GTTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2910.15459481283933826No Hit
CATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2780.14768851535854308No Hit
TTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG2770.14715726170617424No Hit
TTTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2720.14450099344432993No Hit
GGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2700.14343848613959223No Hit
ATTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2660.14131347153011675No Hit
TGTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2630.1397197105730102No Hit
AATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2600.1381259496159036No Hit
ATTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2570.13653218865879702No Hit
TTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2550.1354696813540593No Hit
TATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2450.13015714483037072No Hit
TGTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2400.1275008765685264No Hit
CCTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2370.12590711561141982No Hit
GTTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2350.1248446083066821No Hit
CTTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2340.12431335465431326No Hit
TTTCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2310.12271959369720667No Hit
TTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2300.1221883400448378No Hit
ATTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2290.12165708639246896No Hit
TCTCACGGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2280.12112583274010008No Hit
GTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2180.1158132962164115No Hit
TGTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2160.11475078891167378No Hit
GTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2160.11475078891167378No Hit
TTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA2110.11209452064982947No Hit
ATTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA2090.11103201334509176No Hit
TTATCCTGACGAGGCCAGGGTTTTCCCAGTCACGACCTTAAACAAAAGTG2070.10996950604035402No Hit
GGTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2070.10996950604035402No Hit
GTTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG2070.10996950604035402No Hit
CGTCACGGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2070.10996950604035402No Hit
CGAGACCGACAACTCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT2060.10943825238798517No Hit
TGTCACGGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT2030.10784449143087858No Hit
GATCACGGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGTTTGGTATA2020.10731323777850973No Hit
TTAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG2020.10731323777850973No Hit
TGTCACGGATGTTGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG1990.10571947682140315No Hit
ATTCACGGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT1930.10253195490719No Hit
ATTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACACTAACCTTTCAGT1920.10200070125482112No Hit
GTTCACGGAAGGAGCCAGGGTTTTCCCAGTCACGACGCCTGATGGATCAA1920.10200070125482112No Hit
TAAGACCGACGAGGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG1910.10146944760245227No Hit
TATCACGGACTCAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT1890.10040694029771453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)