FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004976773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37971
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT1200.31603065497353244No Hit
GTGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT1000.26335887914461037No Hit
GCGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT940.24755734639593374No Hit
CGGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT910.2396565800215954No Hit
GTGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA880.23175581364725711No Hit
GTGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT870.22912222485581102No Hit
TCGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT830.21858786969002658No Hit
AGGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT830.21858786969002658No Hit
AAGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT780.20541992573279605No Hit
TTGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT770.20278633694134998No Hit
GGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA770.20278633694134998No Hit
TGGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT760.2001527481499039No Hit
TTGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT750.19751915935845776No Hit
GGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA740.19488557056701167No Hit
GTGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA740.19488557056701167No Hit
TAGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT730.19225198177556557No Hit
TTGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA730.19225198177556557No Hit
AGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA720.18961839298411945No Hit
ATGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT690.18171762660978116No Hit
AAGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA680.17908403781833504No Hit
AGGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT660.17381686023544282No Hit
GTGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA660.17381686023544282No Hit
CTGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT650.17118327144399675No Hit
TGGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT650.17118327144399675No Hit
TTGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA640.16854968265255063No Hit
GTGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA640.16854968265255063No Hit
CGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA630.16591609386110454No Hit
GCGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT620.1632825050696584No Hit
AGGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA620.1632825050696584No Hit
CGGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT620.1632825050696584No Hit
CCGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT620.1632825050696584No Hit
TGGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT600.15801532748676622No Hit
TTGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT600.15801532748676622No Hit
AAGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA590.15538173869532013No Hit
GGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA590.15538173869532013No Hit
GGGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA590.15538173869532013No Hit
GCGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA590.15538173869532013No Hit
ATGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA580.152748149903874No Hit
TAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG580.152748149903874No Hit
CTGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT580.152748149903874No Hit
GGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT570.1501145611124279No Hit
AGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT560.1474809723209818No Hit
TAGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA560.1474809723209818No Hit
TCGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA560.1474809723209818No Hit
GTGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT550.1448473835295357No Hit
TTGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA550.1448473835295357No Hit
GGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT550.1448473835295357No Hit
GCGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT550.1448473835295357No Hit
TAGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT550.1448473835295357No Hit
CAGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT550.1448473835295357No Hit
GAGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA540.1422137947380896No Hit
AAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA540.1422137947380896No Hit
TAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA540.1422137947380896No Hit
GAGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT540.1422137947380896No Hit
GGGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT540.1422137947380896No Hit
TAGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT530.13958020594664347No Hit
TCGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA530.13958020594664347No Hit
TTGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA530.13958020594664347No Hit
GTGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG530.13958020594664347No Hit
ACGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT520.1369466171551974No Hit
TAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA520.1369466171551974No Hit
ACGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT520.1369466171551974No Hit
GAGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT520.1369466171551974No Hit
ATGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT510.13431302836375128No Hit
GTGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA510.13431302836375128No Hit
GCGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA510.13431302836375128No Hit
TTGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGATAACTTCCAATC510.13431302836375128No Hit
GTGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA490.12904585078085906No Hit
ATGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT490.12904585078085906No Hit
TCGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT490.12904585078085906No Hit
GGGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT490.12904585078085906No Hit
CCGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT490.12904585078085906No Hit
ACGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT490.12904585078085906No Hit
TCGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA480.12641226198941297No Hit
AAGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT480.12641226198941297No Hit
TTGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG480.12641226198941297No Hit
TTGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA480.12641226198941297No Hit
TGGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA470.12377867319796687No Hit
TCGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA470.12377867319796687No Hit
AGGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT470.12377867319796687No Hit
GGGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT470.12377867319796687No Hit
AAGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA460.12114508440652078No Hit
TTGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA460.12114508440652078No Hit
ATGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA460.12114508440652078No Hit
GCGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT460.12114508440652078No Hit
ATGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG460.12114508440652078No Hit
AAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT460.12114508440652078No Hit
GAGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT460.12114508440652078No Hit
AGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT450.11851149561507467No Hit
AAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG450.11851149561507467No Hit
GTGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT450.11851149561507467No Hit
CGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT450.11851149561507467No Hit
CTGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT440.11587790682362856No Hit
CAGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT440.11587790682362856No Hit
GTGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT440.11587790682362856No Hit
GAGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA440.11587790682362856No Hit
GCGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT440.11587790682362856No Hit
AGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA430.11324431803218246No Hit
GCGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA430.11324431803218246No Hit
GTGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA430.11324431803218246No Hit
GAGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT430.11324431803218246No Hit
GAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG430.11324431803218246No Hit
AGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA430.11324431803218246No Hit
ATGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA430.11324431803218246No Hit
GTGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA420.11061072924073635No Hit
AAGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA420.11061072924073635No Hit
GGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT420.11061072924073635No Hit
GAGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA420.11061072924073635No Hit
CTGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGATAACTTCCAATC420.11061072924073635No Hit
GGGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA420.11061072924073635No Hit
GGGTTACGATGCACCCAGGGTTTTCCCAGTCACGACGAAACCACTTCTTT420.11061072924073635No Hit
AGGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA420.11061072924073635No Hit
GGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT420.11061072924073635No Hit
CGGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGATAACTTCCAATC420.11061072924073635No Hit
AGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA410.10797714044929024No Hit
GGGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA410.10797714044929024No Hit
TCGTTACGAAGGAGCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT410.10797714044929024No Hit
TTGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA410.10797714044929024No Hit
GCGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA410.10797714044929024No Hit
TTGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT410.10797714044929024No Hit
CGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT410.10797714044929024No Hit
TAGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA400.10534355165784413No Hit
GCGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT400.10534355165784413No Hit
GCGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG400.10534355165784413No Hit
GTGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGCTAAGATGCCTAA400.10534355165784413No Hit
AAGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA400.10534355165784413No Hit
ATGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT400.10534355165784413No Hit
ATGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT390.10270996286639802No Hit
ATGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT390.10270996286639802No Hit
GTGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT390.10270996286639802No Hit
ATGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA390.10270996286639802No Hit
CGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA390.10270996286639802No Hit
GTGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT390.10270996286639802No Hit
CGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACCTCCGCACAACAGT390.10270996286639802No Hit
TTGTTACGACGAGGCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT390.10270996286639802No Hit
TTGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG380.10007637407495194No Hit
GGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT380.10007637407495194No Hit
GCGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT380.10007637407495194No Hit
TTGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA380.10007637407495194No Hit
ATGTTACGATGTTGCCAGGGTTTTCCCAGTCACGACCACAATCTCCTTCA380.10007637407495194No Hit
CGGTTACGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT380.10007637407495194No Hit
CGGTTACGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA380.10007637407495194No Hit
ACGTTACGACTCAGCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA380.10007637407495194No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)