FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004976777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004976777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52020
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT1130.2172241445597847No Hit
TGGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT930.17877739331026526No Hit
ATGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT920.1768550557477893No Hit
TAGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT810.15570934256055363No Hit
AGGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT770.14801999231064975No Hit
TTTCGAAGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT770.14801999231064975No Hit
TTGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT760.14609765474817377No Hit
TGGCAGAGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT760.14609765474817377No Hit
TTTCGAAGATAAGCCCAGGGTTTTCCCAGTCACGACCCTCACTTGATACC730.14033064206074586No Hit
AAGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT730.14033064206074586No Hit
GTGCAGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG720.13840830449826988No Hit
GTGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT710.13648596693579393No Hit
TTTCGAAGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA710.13648596693579393No Hit
TCGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT680.130718954248366No Hit
GGGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT680.130718954248366No Hit
TGGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA660.12687427912341406No Hit
CCGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT660.12687427912341406No Hit
TTGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA660.12687427912341406No Hit
CTGCAGAGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT660.12687427912341406No Hit
GGGCAGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG650.1249519415609381No Hit
TTGCAGAGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT650.1249519415609381No Hit
TTTCGAAGATAAGCCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG650.1249519415609381No Hit
GAGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT640.12302960399846213No Hit
TGGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT640.12302960399846213No Hit
CTGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT640.12302960399846213No Hit
GTTCGAAGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT640.12302960399846213No Hit
TGGCAGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG640.12302960399846213No Hit
CGGCAGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG630.12110726643598616No Hit
TTTCGAAGACAACTCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA620.11918492887351018No Hit
TTTCGAAGACGAGGCCAGGGTTTTCCCAGTCACGACCCAAGTTGGATGAG620.11918492887351018No Hit
GTGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA610.11726259131103421No Hit
GCGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT600.11534025374855825No Hit
TAGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT600.11534025374855825No Hit
TAGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA600.11534025374855825No Hit
CTGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT600.11534025374855825No Hit
TATCGAAGACAACTCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA590.11341791618608228No Hit
CGGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT590.11341791618608228No Hit
ACGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT580.11149557862360629No Hit
GTGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT580.11149557862360629No Hit
ATGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA570.10957324106113032No Hit
GGGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT560.10765090349865436No Hit
CGGCAGAGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT560.10765090349865436No Hit
CAGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACAGGTCGTTTTTCTT560.10765090349865436No Hit
GCGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT550.1057285659361784No Hit
TTTCGAAGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA550.1057285659361784No Hit
GTTCGAAGATAAGCCCAGGGTTTTCCCAGTCACGACCCTCACTTGATACC550.1057285659361784No Hit
AGGCAGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG550.1057285659361784No Hit
GTTCGAAGAGGAATCCAGGGTTTTCCCAGTCACGACGATTAATTGCCACA540.10380622837370243No Hit
AAGCAGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG540.10380622837370243No Hit
TGGCAGAGAGGAATCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT540.10380622837370243No Hit
ATTCGAAGATGCACCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT540.10380622837370243No Hit
GAGCAGAGAAGGAGCCAGGGTTTTCCCAGTCACGACGACATTCGTTCAAA530.10188389081122644No Hit
TAGCAGAGACAACTCCAGGGTTTTCCCAGTCACGACGCTGAAAGGACTGG530.10188389081122644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)