FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8120528
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN397320.4892785296719622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG45300.014.6455945
CTGTCGC13950.014.0020869
CGTATAC4002.8012437E-1013.4709713
TGTCGCC14050.013.18333510
CTATACT30850.012.7723164
TATGCGC1900.002529640412.40747410
GCGTAGA2752.0934802E-512.2463381
GTATACG3601.7660386E-712.1612941
TACACTG35500.012.0479825
CCTACGC2802.5180841E-512.0276543
TCTGTCG14650.011.9537648
GTCGTAG2307.906562E-411.7138891
TTATACT48500.011.5961464
TCTATAC27750.011.5292113
GTACTAG12450.011.3610611
CTACACT26700.011.3519424
GTCGCCC11600.011.32258411
CTAATAC28400.011.2653383
ATACTGT58950.011.0258596
TACCCCG2752.8387835E-411.0217045
Produced by FastQC (version 0.11.5)