FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004977233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8120528
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN386320.47573261246066756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA3554.629328E-918.66990570
CTGTCGC13100.014.1475659
GTCTCGC13250.013.9874041
ATCGTCG1450.00610047313.9435568
GTCGCCC11700.013.8243811
TGCGCGT1750.001356827913.4787710
TGTCGCC13750.013.4787710
GTATAGG15200.013.0797271
TACACCG2651.4226098E-512.7158215
CTCGCTC14300.012.4890713
TCGCTCT13700.012.2981484
TCTGTCG13300.012.1612978
TCGCCCA14050.011.99178812
GTGTAGG21400.011.80966951
TATACTG45650.011.7367175
GTCGTAA2601.6775423E-411.6643211
ACTACGC4351.3847966E-811.619633
AGTGCGC2050.00444742311.5062688
TACGACC2050.00444742311.5062684
TCTCGCT17650.011.4550462
Produced by FastQC (version 0.11.5)