FastQCFastQC Report
Fri 27 May 2022
EGAF00004977253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004977253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379889490
Sequences flagged as poor quality0
Sequence length74
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCT12224620.3217941091236823No Hit
GGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACA11211030.2951129287625199No Hit
GTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAAT11044570.2907311281499259No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTA10989920.28929255189449965No Hit
GCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTT10901100.28695450353206664No Hit
GCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGC7510940.19771381408840769No Hit
GCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTA7204430.18964541503898935No Hit
AGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCT7114570.18727999029401945No Hit
GTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCC6819100.1795022020746086No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCT6712230.17668901553449134No Hit
GGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAG6658080.1752636010014386No Hit
CCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGT6463900.17015211450045645No Hit
CTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACA6438660.1694877107550409No Hit
GCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGC6317050.16628651663935215No Hit
GCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAG6212650.16353834900776013No Hit
CTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGT6185930.16283498656411893No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGC6119860.16109579656968137No Hit
GAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGG6078230.15999995156486166No Hit
GTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCC5905600.1554557352981784No Hit
CACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCC5900380.15531832691659883No Hit
GCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAAC5816810.15311847663908787No Hit
AAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAG5604360.1475260608025771No Hit
AGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGAT5576810.14680084989979586No Hit
GGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGG5456250.1436272954010915No Hit
GGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCC5334980.14043505125661676No Hit
GGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGA5226350.1375755354537447No Hit
AGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA5208860.13711513840511882No Hit
ATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCA5207770.13708644585034455No Hit
GCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAG5193830.13671949703057065No Hit
GCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCC5095720.13413690386643756No Hit
GTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGA4891270.1287550755879032No Hit
AAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGGC4850060.12767028642987727No Hit
GCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGA4656950.12258696601477445No Hit
GGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGC4617550.12154982229174069No Hit
GAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTC4585950.12071800143773391No Hit
CACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTC4506610.11862949933150296No Hit
GAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGC4416090.11624670111299998No Hit
GGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACC4372790.11510689595545273No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACA4348240.11446065538691266No Hit
CTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAA4296550.11309999652793763No Hit
GAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAG4271720.1124463853948684No Hit
GAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGG4258350.11209444093860033No Hit
GTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAG4255170.11201073238430471No Hit
ACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA4159360.10948868314308985No Hit
GCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTG4149990.10924203246580999No Hit
AGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAG4133120.10879795595292725No Hit
GTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGG4129800.10871056211636705No Hit
CTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAG4065420.10701585874355198No Hit
GACAGTGGCCCCTACAGAATGTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATC4059080.10684896810385569No Hit
CAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTAA4032870.1061590306170355No Hit
GTTCATAGGTTCTAAACCCTCACCCCCCCCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCT3971280.10453776965506469No Hit
GGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCT3890750.10241794264958476No Hit
GGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGA3799480.10001540184752149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)